mouse anti-tff2  (Abnova)

Journal: PLoS ONE

Article Title: Targeted Deletion of Kcne2 Causes Gastritis Cystica Profunda and Gastric Neoplasia

doi: 10.1371/journal.pone.0011451

Figure Lengend Snippet: A. Left , Negative control for TFF2 staining in areas of GCP from a Kcne2 −/− mouse, using IgM isotype. Scale bar, 70 µm. Right , TFF2 staining in areas of GCP from a Kcne2 −/− mouse, using IgG isotype. Scale bar, 50 µm. B. TFF2 staining in cystic region of gastric mucosa from a Kcne2 −/− mouse. Scale bar, 50 µm. C. Increased magnification views of TFF2 staining in gastric mucosa from a Kcne2 −/− mouse. Left , gastric gland cross-section; right , gastric gland longitudinal section. Scale bar, 50 µm.

Article Snippet: The primary antibody concentrations used were: 0.05 µg/ml (rabbit polyclonal anti-Ki67, Vector Labs); 1 µg/ml (mouse monoclonal anti-CK-7; Abcam); 1 µg/ml (mouse monoclonal anti-TFF2; Abcam).

Techniques: Negative Control, Staining

Journal: International Journal of Molecular Sciences

Article Title: Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid Arthritis

doi: 10.3390/ijms20236105

Figure Lengend Snippet: Presence and localization of TFFs in synovial membrane (SM). ( A ) Semi-quantitative PCR analysis of TFFs mRNA in human SM of five healthy (28, 48, 54, 78, and 92 years (Lines 1, 2, 3, 4, and 5)), rheumatoid arthritis (RA) (Lines 6 and 7) and osteoarthritis (OA) (Lines 8 and 9) samples. Line 10 represents the negative control (without cDNA template), and human stomach serves as positive control (Line 11). Beta-actin (β-actin) was used as the loading control. ( B ) Immunohistochemical analysis of TFF1, -2 and -3 in human SM of 22 ( a , b , c ), 48 ( d , e , f ), and 83 ( g , h , i ) year-old healthy donors. TFF3 is present in each examined sample ( c , f , i ). TFF1 ( a , d , g ) and TFF2 ( b , e , h ) reveal negative results irrespective of the donor’s age. Insets show magnifications. Scale bars: 100 μm. Red staining indicates positive antibody reaction.

Article Snippet: mouse anti-TFF2 , WB , monoclonal , H00007032-M01, Abnova.

Techniques: Real-time Polymerase Chain Reaction, Negative Control, Positive Control, Immunohistochemical staining, Staining

Journal: International Journal of Molecular Sciences

Article Title: Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid Arthritis

doi: 10.3390/ijms20236105

Figure Lengend Snippet: Detection and quantification of TFF peptides in synovial fluid (SF). ( A ) Western blot analysis of TFFs in human SF of healthy (Line 1 and 2), osteoarthritis (OA; Line 3 and 4), and rheumatoid arthritis (RA; Line 5 and 6) samples. Proteins of the human stomach serving as positive control (Line 7) were included in the test. Actin (≈ 43 kDa) and alpha-1-antitrypsin (≈ 51 kDa) were used as loading control. Molecular weight marker is shown on the right. ELISAs of TFF1 ( B ), –2 ( C ), and –3 ( D ) in human SF of healthy ( n = 13), OA ( n = 20) and RA ( n = 20) samples. Mean values are: TFF1: 50.87 pg/mg (healthy), 31.16 pg/mg (OA), and 33.04 pg/mg (RA); TFF2: 13.69 pg/mg (healthy), 8.97 pg/mg (OA), 203.50 pg/mg (RA); TFF3: 7807.29 pg/mg (healthy), 2833.90 pg/mg (OA), 4083.92 pg/mg (RA). The protein concentration is expressed in pg/mg and visualized as mean value and standard error of the mean (SEM). * indicates significant differences performing the Mann–Whitney U -Test (significance level p ≤ 0.05).

Article Snippet: mouse anti-TFF2 , WB , monoclonal , H00007032-M01, Abnova.

Techniques: Western Blot, Positive Control, Molecular Weight, Marker, Protein Concentration, MANN-WHITNEY

Journal: International Journal of Molecular Sciences

Article Title: Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid Arthritis

doi: 10.3390/ijms20236105

Figure Lengend Snippet: ELISA quantification of TFF2 in synovial fluid.

Article Snippet: mouse anti-TFF2 , WB , monoclonal , H00007032-M01, Abnova.

Techniques: Enzyme-linked Immunosorbent Assay, Protein Concentration

Journal: International Journal of Molecular Sciences

Article Title: Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid Arthritis

doi: 10.3390/ijms20236105

Figure Lengend Snippet: Primers used for semi-quantitative PCR and real-time PCR.

Article Snippet: mouse anti-TFF2 , WB , monoclonal , H00007032-M01, Abnova.

Techniques: Real-time Polymerase Chain Reaction

Journal: International Journal of Molecular Sciences

Article Title: Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid Arthritis

doi: 10.3390/ijms20236105

Figure Lengend Snippet: Primary and secondary antibodies used for western blot (WB) and immunohistochemistry (IHC)

Article Snippet: mouse anti-TFF2 , WB , monoclonal , H00007032-M01, Abnova.

Techniques: Western Blot, Immunohistochemistry, Plasmid Preparation

Journal: Cancer gene therapy

Article Title: Therapeutic potential of adenovirus-mediated TFF2-CTP-Flag peptide for treatment of colorectal cancer.

doi: 10.1038/s41417-018-0036-z

Figure Lengend Snippet: Fig. 3 Validation of Ad-Tff2-CTP-Flag activity in AOM/DSS-induced colon cancer model. a, b Ad-Tff2 suppresses colon tumorigenesis in wild- type mice treated with AOM/DSS. a Colon appearance (left), tumor number (right), b number of splenic CD11b+Gr-1+ cells. Two experiments have been done with 3–4 mice in each group. c, d Ad Tff2-CTP-Flag suppresses colon tumorigenesis in AOM/DSS-induced colon cancer model. c Colon appearance and tumor number, d proportion CD11b+Gr-1+ cells in the spleen from wild-type mice treated with Ad-Tff2-CTP versus Ad- Fc-CTP, unpaired t-test, *p < 0.05. Two experiments have been done with 3–4 mice in each group. e Overlapping staining for Flag and Gr-1 in the spleen of Tff2-null mice injected with Ad-Tff2-CTP-Flag. Mice were given 2% DSS for 5 consecutive days, then seven days later adenovirus was administrated via tail vein and mice were sacrificed in one week after adenovirus administration. Bar size is 50 µm. f BrdU incorporation in splenic CD11b+Gr-1+ cells of wild-type mice injected with Ad-Fc-CTP-Flag compare with Ad-Tff2-CTP-Flag, unpaired t-test. Wild-type mice were treated 2% DSS for 5 consecutive days, then on day 7 post treatment with DSS single injection of Ad-Tff2-CTP or Ad-Fc-CTP-Flag (both 5 × 108

Article Snippet: All blots were blocked in 5% skim milk in Tris-buffered saline (TBS) plus Tween 20 (0.05%) for 1 h. Affinitypurified primary polyclonal rabbit antibodies were produced against the C-terminus of mouse TFF2 (New England Peptide) and used at concentration 1 μg ml−1.

Techniques: Biomarker Discovery, Activity Assay, Staining, Injection, BrdU Incorporation Assay

Journal: Scientific Reports

Article Title: Nardilysin regulates inflammation, metaplasia, and tumors in murine stomach

doi: 10.1038/srep43052

Figure Lengend Snippet: ( A ) Representative macroscopic views of the stomachs of Nrdc + / + and Nrdc −/− mice. ( B ) H&E staining of Nrdc + / + and Nrdc −/− mouse stomachs. Bars = 100 μm. ( C ) Immunohistochemistry for pepsinogen II, H + /K + -ATPase, Muc5ac, TFF2, and Ki67 in Nrdc + / + and Nrdc −/− mice. Bars = 100 μm. ( D ) Percentages of epithelial cells immunostained with pepsinogen II, H + /K + -ATPase, Muc5ac, TFF2, and Ki67 in Nrdc + / + and Nrdc −/− mice.

Article Snippet: The primary antibodies used were rat anti-F4/80 (Abcam, Cambridge, MA, USA), rat anti–Gr-1 (eBioscience, San Diego, CA, USA), sheep anti-pepsinogen II (Abcam), mouse anti-H + /K + -ATPase α subunit (MBL, Nagoya, Japan), mouse anti-Muc5AC (Abcam), mouse-anti-spasmolytic polypeptide (TFF2) (R&D Systems, Minneapolis, MN, USA), and rat anti-Ki67 (Dako, Glostrup, Denmark).

Techniques: Staining, Immunohistochemistry

Journal: Scientific Reports

Article Title: Nardilysin regulates inflammation, metaplasia, and tumors in murine stomach

doi: 10.1038/srep43052

Figure Lengend Snippet: ( A )Immunohistochemistry for TFF2 in Nrdc + / + and Nrdc −/− mouse stomachs with Helicobacter felis infection. Bars = 100 μm. ( B ) Areas stained for TFF2 in Nrdc + / + and Nrdc −/− mouse stomachs with Helicobacter felis infection. *P < 0.05. ( C ) Alcian blue staining of Nrdc + / + and Nrdc −/− mouse stomachs with Helicobacter felis infection. Bars = 100 μm. ( D ) Areas stained with Alcian blue in Nrdc + / + and Nrdc −/− mouse stomachs with Helicobacter felis infection. *P < 0.05. ( E ) Alcian blue staining of Nrdc + / + and Nrdc −/− mouse stomachs with PGE 2 expression. Bars = 1000 μm. ( D ) Areas stained with Alcian blue in Nrdc + / + and Nrdc −/− mouse stomachs with forced PGE 2 expression. *P < 0.05.

Article Snippet: The primary antibodies used were rat anti-F4/80 (Abcam, Cambridge, MA, USA), rat anti–Gr-1 (eBioscience, San Diego, CA, USA), sheep anti-pepsinogen II (Abcam), mouse anti-H + /K + -ATPase α subunit (MBL, Nagoya, Japan), mouse anti-Muc5AC (Abcam), mouse-anti-spasmolytic polypeptide (TFF2) (R&D Systems, Minneapolis, MN, USA), and rat anti-Ki67 (Dako, Glostrup, Denmark).

Techniques: Immunohistochemistry, Infection, Staining, Expressing